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. 2002 Jun;184(11):2863–2869. doi: 10.1128/JB.184.11.2863-2869.2002

FIG. 5.

FIG. 5.

Western blot of PtlF expression for the wild type and PtlE mutants. The ptx/ptl operon containing wild-type PtlE or point mutants was introduced into the chromosome of pertussis toxin deletion strain BPRA at the adneylate cyclase toxin locus. Expression of PtlF was assessed to monitor polarity. Lane 1, BPRA; lane 2, BPRA with wild-type (WT) PtlE (from pKC34); lane 3, BPRA with PtlE D53A (from pAR287-2); lane 4, BPRA with PtlE E62A (from pAR264-3); lane 5, BPRA PtlE D53A E62A (from pAR264-4).