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. 2002 Jun;184(11):2863–2869. doi: 10.1128/JB.184.11.2863-2869.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 7. — Toxin expression and secretion in merodiploid strains (wild-type background). PTWT, BP338 with a second copy of wild-type PtlE (from pKC34); D53A, BP338 with a second copy of mutant PtlE from pAR287-2; E62A, BP338 with a second copy of mutant PtlE from pAR264-3; D53A+E62A, BP338 with a second copy of mutant PtlE from pAR264-4. Cell-associated (periplasmic) and secreted toxins were quantified by using the CHO cell activity assay. Periplasmic and secreted toxin levels were not significantly different for any of the strains tested.