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Activity of polyhistidine-tagged mutant PtlE proteins expressed in E. coli BL21[DE3]. Lane1, vector control (pET-21b); lane 2, His-tagged PtlE wild type (pAR312-1); lane 3, His-tagged PtlE D53A (pAR312-2); lane 4, His-tagged PtlE E62A (pAR312-3); lane 5, His-tagged PtlE double mutant (pAR312-4). (A) Western blot analysis with anti-polyhistidine antibody; (B) zymogram analysis.
