TABLE 2.
Primers used
| Primer | Sequencea |
|---|---|
| 5-F80 | GGCTCTAGAGACGGCTGGCAATTCAGCC |
| 3-F80 | CAGAAGCTTACCCGGTCTGAACGTGAGCC |
| 5-E180 | GGCTCTAGAATGGGCCATCCTGGCCATC |
| 3-E280 | CAGAAGCTTCATGGCTGTCCAGCCTCCG |
| 5-HSP | GAGCTCGCGAAGACCCGCC |
| 3-HSP | CCGCGGATGAGGAACTCCTG |
| 5-AR156 | CTTGACGCCTGCCCAGACGC |
| 3-AR156 | GTCTGGGCAGGCGTCAAGGGG |
| 5-AR264 | GCCGCGGCCGCGGTGGAC |
| 3-AR264 | GCGGCCGCGGCATGCCCG |
| 3-AR301 | GAAGCTTCACCATGCACGGCGTTCCGAC |
| 5-D80 | GGCTCTAGAGCGGGCCTGCGGCG |
a
The underlined nucleotides are the nucleotides altered to produce the amino acid substitutions.