Biosynthetic pathway of the compatible solute ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) based on enzymological and genetic studies (14, 29, 40). 1, l-2,4-Diaminobutyric acid transaminase (encoded by ectB); 2, l-2,4-diaminobutyric acid Nγ-acetyltransferase (encoded by ectA); 3, ectoine synthase (encoded by ectC).