TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Sourceb or reference |
|---|---|---|
| H. elongata | ||
| DSM 2581T | Type strain | DSMZ |
| KB1 | ΔectA Ect− | This study |
| AFE35 | ΔectA teaC::Tn1732 Kmr Ect−; osmoregulatory ectoine uptake deficiency | This study |
| KB1-2 | ΔectA ΔteaB Ect−; osmoregulatory ectoine uptake deficiency | This study |
| KB1-3 | ΔectA ΔteaA Ect−; osmoregulatory ectoine uptake deficiency | This study |
| KB1-4 | ΔectA ΔteaC Ect−; osmoregulatory ectoine uptake deficiency | This study |
| KB1-5 | ΔectA Δ(orf1::Ω) Smr Spr Ect− | This study |
| KB2 | ΔteaC; osmoregulatory ectoine uptake deficiency, ectoine excretion | This study |
| KB2-1 | ΔteaCB; osmoregulatory ectoine uptake deficiency, ectoine excretion | This study |
| E. coli | ||
| K-12 | Wild type | DSMZ |
| DH5α | F− φ80dlacZDM15 Δ(lacZYA-argF)U169 recA1 hsdR17(rK− mK+) supE44 Δ−thi-1 gyrA relA1 | 17 |
| S17-1 | thi pro hsdR hsdM+recA Tpr Smr | 49 |
| SM10 | Kmr Thi Pro His | 49 |
| Plasmids | ||
| pSUP102-Gm::Tn1732 | Kmr Cmr | U. Priefer, University of Bielefeld |
| pK18mobsacB | Kmrmob sacB | 47 |
| pKGB1 | 1,043-bp PCR fragment for ectA deletion containing 510 bp upstream and 533 bp downstream of ectA, Kmr | This study |
| pKGB2 | 1,346-bp PCR fragment for teaB deletion containing 671 bp upstream and 675 bp downstream of teaB, Kmr | This study |
| pKGB3 | 1,863-bp PCR fragment for teaA deletion containing 940 bp upstream and 923 bp downstream of teaA | This study |
| pAVB1 | 2,197-bp PCR fragment for teaC deletion containing 1,090 bp upstream and 1,107 bp downstream of teaC, Kmr | This study |
| pKGB4 | 1,025-bp PCR fragment for orf1 deletion containing 512 bp upstream and 513 bp downstream of orf1 with Ω cassette insertion, Spr Smr Kmr | This study |
| pKGB6 | 1,045-bp PCR fragment for teaBC deletion containing 493 bp upstream and 552 bp downstream of teaBC | This study |
a
Abbreviations: Cm, chloramphenicol; Km, kanamycin; Sm, streptomycin; Tp, trimethoprim; Ect, ectoine.
b
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.