TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| W3110 (wild type) | lacL8 lacIq | Laboratory strain |
| AK15 | W3110 trp::putPA1303::[Kanr-astC′-′lac] | This study |
| AK16 | W3110 trp::putPA1303::[Kanr-astC′-′lac] rpoS::tet | This study |
| AK17 | W3110 trp::putPA1303::[Kanr-astC′-′lac] himD::cam | This study |
| AK18 | W3110 trp::putPA1303::[Kanr-astC′-′lac] argR::cam | This study |
| AK21 | W3110 trp::putPA1303::[Kanr-astC′-′lac] Δcrp Strr | This study |
| AK22 | W3110 rpoN::FRT-Kanr-FRT | This study |
| AK23 | W3110 ΔrpoN | This study |
| AK24 | W3110 trp::putPA1303::[Kanr-astC′-′lac] ΔrpoN | This study |
| AK26 | W3110 trp::putPA1303::[Kanr-astC′-′lac] ΔrpoN rpoS::tet | This study |
| CA8445 | Δcrp Strr | 37 |
| K5746 | Overproduces both IHF subunits | 30 |
| TE2680 | Δ(lac)X74 recD1903::Tn10 trpDC700::putPA1303::[Kans-Camr-′lac] | 13 |
| K4633 | recD::tet | D. Friedman (University of Michigan) |
| BW12848 | himD::cam | B. Wanner (Purdue University) |
| UM315 | rpoS::tet | Laboratory strain originally obtained from P. C. Loewen |
| Plasmids | ||
| pRS551 | Ampr Kanr-′lac | 42 |
| pTE103 | Ampr | 14 |
| pAK10 | AmprastCp | This study |
| pUTmini-Tn5 Cm | Ampr MCS-cam-MCS | 10 |
| pQErpoS30 | Ampr ptac::His6-rpoS | 3 |
| pTH7 | Ampr ptac::rpoN | 19 |
| pXZCRP | Amprcrp | 51 |