TABLE 3.
Virulence of P. aeruginosa efflux mutants in a rat model of acute pneumonia
| Strain | Inoculum (106 CFU) | n | % Mortalitya | P | Time to death (days) | Lung wt (g)b | Bacterial counts in lungs (log CFU/g) |
|---|---|---|---|---|---|---|---|
| PT5 (wild type) | 3.0 | 22 | 72 | 1.6 ± 0.63 | 3.98 ± 0.50 | 8.30 ± 0.54 | |
| PT625 (NalC) | 1.7 | 18 | 50 | 0.19 | 2.1 ± 1.0 | 3.84 ± 0.9 | 8.53 ± 0.38 |
| PT648 (nfxB) | 2.1 | 11 | 45 | 0.15 | 1.6 ± 0.89 | 4.83 ± 1.35 | 8.85 ± 0.28 |
| PT149 (NfxC) | 2.1 | 18 | 0 | <0.001 | NRc | NDd | ND |
| PT637 (NfxC, mexE) | 0.9 | 12 | 42 | 0.006 | 2.8 ± 1 | 4 ± 1.21 | 8.44 ± 0.48 |
a
Statistical significance (P < 0.05) was evaluated with a Student's t test calculated versus PT5, except for PT637, which was calculated against PT149.
b
Lung weight of uninfected rat, 1.0 ± 0.2 g.
c
NR, not relevant.
d
ND, not determined.