TABLE 2.
Activity of IntI3 recombinase
| Recombination site | Fragment lengtha (nt) | Plasmidb | Mean cointegration frequency (no. of assays)
|
||||
|---|---|---|---|---|---|---|---|
| pMAQ487 (Flag-IntI3) | pRMH851 (IntI3) | pRMH505 (Flag-IntI1) | pRMH850 (IntI1) | No integrase | |||
| aadB/qacE | 202/198 | pMAQ28 | 1.5 × 10−4 (10) | —c | 1.3 × 10−3 (6) | 1.8 × 10−3 (4) | 9.5 × 10−8 (3) |
| dfrA7/qacE | 221/755 | pRMH555 | 7.0 × 10−4 (6) | — | — | — | — |
| aacA4/qacE | 228/198 | pMAQ636 | 3.9 × 10−3 (6) | — | — | — | — |
| attI3/qacE | 284/198 | pRMH866 | — | 2.2 × 10−3 (5) | — | — | — |
| attI3/qacE | 131/198 | pMAQ545 | 7.1 × 10−4 (6) | 1.4 × 10−3 (5) | — | — | 2.1 × 10−7 (3) |
| attI3/blaIMP-1 | 131/34 | pMAQ484 | 8.1 × 10−4 (5) | 8.4 × 10−4 (4) | — | — | — |
| attI1/qacE | 64/198 | pRMH251 | — | — | 1.1 × 10−2 (7) | 8.6 × 10−3 (6) | 3.4 × 10−7 (4) |
| None | pACYC184 | — | 5.0 × 10−7 (6) | — | 1.3 × 10−5 (6) | — | |
a
Number of nucleotides (nt) in the cloned fragment to the left/right of the recombination crossover point.
b
Cells also contained pMAQ495.
c
—, not determined.