TABLE 3.
Nucleotide sequences surrounding secondary recombination sites in pACYC184
| pACYC184 sequencea | Positionb (strand) | pMAQ495 recombination site | No. of independent isolates |
|---|---|---|---|
| CCTTAGCTCCTGAAAATCTC GAT AACTCAAAAAATACGCCCGG | 248 (+) | orfA | 1 |
| CTACCGGGCGTATTTTTTGA GTT ATCGAGATTTTCAGGAGCTA | 253 (−) | dfrB2 | 1 |
| AATGAGACGTTGATCGGCAC GTA AGAGGTTCCAACTTTCACCA | 312 (−) | orfA | 1 |
| ATCATACACTAAATCAGTAA GTT GGCAGCATCACCCGACGCAC | 457 (−) | dfrB2 | 1 |
| TTCTATCAGCTGTCCCTCCT GTT CAGCTACTGACGGGGTGGTG | 529 (+) | dfrB2 | 1 |
| CGCGGCCCTCTCACTTCCCT GTT AAGTATCTTCCTGGCATCTT | 816 (−) | orfA | 1 |
| CGCGGCCCTCTCACTTCCCT GTT AAGTATCTTCCTGGCATCTT | 816 (−) | dfrB2 | 2 |
| CGATAAGCTTTAATGCGGTA GTT TATCACAGTTAAATTGCTAA | 1539 (+) | orfA | 1 |
| TAATGCGGTAGTTTATCACA GTT AAATTGCTAACGCAGTCAGG | 1549 (+) | orfA | 2 |
| AGGATGACGATGAGCGCATT GTT AGATTTCATACACGGTGCCT | 1591 (−) | orfA | 2 |
| AGCCGCGAGCGATCCTTGAA GCT GTCCCTGATGGTCGTCATCT | 2585 (−) | orfA | 1 |
| ATAAAAACGGTTAGCGCTTC GTT AATACAGATGTAGGTGTTCC | 3497 (−) | orfA | 1 |
| TTGGTGCCCTTAAACGCCTG GTT GCTACGCCTGAATAAGTGAT | 3767 (−) | dfrB2 | 1 |
a
Nucleotides in bold type are those to which recombination crossover can be confined.
b
Numbers indicate the position of the G residue in the central triplet in the pACYC184 sequence (51) (modified according to accession no. X06403). The T at position 3766 is replaced by a TT in the pACYC184 used here. + indicates the strand shown in the published sequence, and − indicates the complementary strand.