FIG. 5.
Promoter mapping of the ggpS gene by primer extension analysis (A) and comparison of the ggpS promoter with promoter sequences of genes with known salt-dependent expression from Synechocystis, E. coli, and B. subtilis (B). (A) For the sequencing reaction (lanes A, C, G, and T) and cDNA synthesis (lanes Co and SS) the same primer was used. Total RNA was isolated from cells grown under control conditions (lane Co) or from cells shocked for 2 h by 684 mM NaCl (lane SS). (B) Promoter sequences are indicated by the names of the genes, as follows: ggpS (this study); slr0081, slr0082, and isiA, encoding proteins of unknown function and iron stress-induced protein A (25); E. coli σ70, the consensus sequence for σ70-dependent promoters; betT and betIBA, encoding choline uptake system BetT (15); proP P1 and proU, encoding proline uptake systems (18); opuE P1 σA and opuE P2 σB, encoding proline uptake systems (24); and opuB, encoding a choline uptake system (14).