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Accumulation of ppGpp and pppGpp in E. coli CF1652 (ΔrelA) containing pBAD18 (vector control) (lane 1), BADpo NCH 1-739 (full-length RelSeq) (lane 2), pBAD NH 1-385 (lane 3), BADpo NH 79-385 (lane 4), and BADpo NH 1-224 (lane 5). Resolved nucleotides were extracted from uniformly 32P-labeled cultures grown under steady-state conditions in 0.02% arabinose. The radioactive spot near the origin in lane 5 is not reproducible and not identified.
