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The RelA C-terminal domain restores (p)ppGpp accumulation response to amino acid starvation in the RelSeq/RelA chimera. Cultures were grown and uniformly 32P labeled in MOPS containing 0.002% arabinose for low-level protein induction. Serine hydroxamate was added at time zero. The strains tested were CF4941 derivatives containing pLB15 (RelA), pLB18 (RelA/RelSeq), pLB16 (RelSeq), and pLB19 (RelSeq/RelA).
