Figure 4.—

Gel mobility shifts using the ovo-B core promoter and extracts from gonadal and somatic tissues. (A) Shifting activities from proteins extracted from female or male gonads or nongonadal soma were used. The source of protein for the gel shifts is indicated above the lane. Bands highly enriched in shifts using ovarian extracts (open arrow) or testis (solid arrow) are indicated. (B) Similar gel shifts performed with increasing amounts of unlabeled wild-type OVO binding site oligo or mutated oligo. The mobility of the shifted band in the absence of competitor is indicated (arrow).