TABLE 5.
Influence of EIIAGlc overexpression on repression of malT-lacZ by G3P
Strain | Relevant genotype | IPTG concn [μM] | β-Galactosidase sp acta
|
|
---|---|---|---|---|
TB | TB-G3P | |||
Bre1161 | 0.43 | 0.18 | ||
Bre1161/pBCP260 | prrp+ | 0 | 0.43 | 0.20 |
10 | 0.43 | 0.22 | ||
30 | 0.37 | 0.27 | ||
ET18 | glpD glpR | 0.39 | 0.15 | |
ET18/pBCP260 | glpD glpR prrp+ | 0 | 0.43 | 0.20 |
10 | 0.41 | 0.19 | ||
30 | 0.36 | 0.19 | ||
ET16 | glpK glpF | 0.40 | 0.15 | |
ET16/pBCP260 | glpK glpF crrp+ | 0 | 0.42 | 0.21 |
10 | 0.39 | 0.19 | ||
30 | 0.38 | 0.18 |
Specific activity is given in units per milligram of protein. Strains were grown overnight at 37°C in TB or in TB-0.4% G3P.