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. 2002 Jun;184(11):3044–3052. doi: 10.1128/JB.184.11.3044-3052.2002

TABLE 5.

Influence of EIIAGlc overexpression on repression of malT-lacZ by G3P

Strain Relevant genotype IPTG concn [μM] β-Galactosidase sp acta
TB TB-G3P
Bre1161 0.43 0.18
Bre1161/pBCP260 prrp+ 0 0.43 0.20
10 0.43 0.22
30 0.37 0.27
ET18 glpD glpR 0.39 0.15
ET18/pBCP260 glpD glpR prrp+ 0 0.43 0.20
10 0.41 0.19
30 0.36 0.19
ET16 glpK glpF 0.40 0.15
ET16/pBCP260 glpK glpF crrp+ 0 0.42 0.21
10 0.39 0.19
30 0.38 0.18
a

Specific activity is given in units per milligram of protein. Strains were grown overnight at 37°C in TB or in TB-0.4% G3P.