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Transcriptional response of chaperone genes to accumulation of misfolded polypeptides. (A) Primer extension (Pgro, Phrc) and S1 nuclease (Pcbp) analyses of H. pylori RNA extracted from cells grown at 37°C (0 min) (lane 1) or upon the addition of puromycin (lanes 2 to 10). Symbols are as described in the legend to Fig. 2. (B) Pattern of RNA accumulation at the indicated promoters as obtained by PhosphorImager analysis quantifications of the radioactive bands shown in panel A.
