TABLE 3.
Biochemical properties of subunits of acetone carboxylases from R. capsulatus B10 and X. autotrophicus Py2
| Acetone carboxylase source | Biochemical properties of subunit:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| α
|
β
|
γ
|
|||||||
| Molecular mass (kDA) by:
|
Relative molar ratiob | Molecular mass (kDA) by:
|
Relative molar ratio | Molecular mass (kDA) by:
|
Relative molar ratio | ||||
| MALDI-TOF (MS) | SDS-PAGE | MALDI-TOF (MS) | SDS-PAGE | MALDI-TOF (MS) | SDS-PAGE | ||||
| R. capsulatus B10 | 85.2 | 82 | 1.0 | 78.6 | 70 | 1.1 | 19.5 | 19 | 1.2 |
| X. autotrophicus Py2a | 85.3 | 79 | 1.0 | 78.3 | 68 | 1.0 | 19.6 | 23 | 1.2 |
a
Data are from Sluis and Ensign (42).
b
Determined by integrated scanning densitometry.