TABLE 4.

Identification and characterization of acetone carboxylase genes and gene products

Bacterium and parameter	Result for subunit:
	α	β	γ
R. capsulatus B10
N terminus (Edman degradation)	LNAPTAIRGIV	LDREKTRSVQ	AYTKAKIKDLV
N terminus (deduced)	MNAPTAIRGIVR	MPLDREKTRSVQ	MAYTKAKIKDLV
Mr (deduced)	85,319	78,936	19,639
No. of amino acids	769	717	167
Gene designationa	RRC02651	RRC02652	RRC02650
X. autotrophicus Py2
N terminus (Edman degradation)	NDb (blockedc)	ND (blocked)	AYTXAKIVDLVDG
N terminus (deduced)	MNVTVDQSTLAG	MNVPVGHLRNVQ	MAYTRSKIVDLVDG
Mr (deduced)	86,342	78,509	19,774
No. of amino acids	776	717	168
Gene designationd	acxB	acxA	acxC
H. pylori 26695
N terminus (Edman degradation)	ND	ND	ND
N terminus (deduced)	MANLLKNGKTL	MKDARVQVMGID	MVMSKYTQEQI
Mr (deduced)	86,511	78,532	19,915
No. of amino acids	765	713	168
Gene designatione	HP0696	HP0695	HP0697
H. pylori J99
N terminus (Edman degradation)	ND	ND	ND
N terminus (deduced)	MANLLKNGKTLK	MKDARVQVMGID	MSKYTQEQIKNL
Mr (deduced)	86,607	78,332	19,670
No. of amino acids	765	712	166
Gene designatione	jhp0632	jhp0633	jhp0631
Alignment data
% Residues conserved (all four)	61.3	62.7	49.7

^aGene designations as assigned by the Capsulapedia sequencing project (50).

^bND, not determined.

^cBlocked, the N terminus could not be sequenced due to apparent blockage.

^dThe genes from X. autotrophicus have been deposited in GenBank under accession no. AY055852.

^eGene designations as assigned by the genome sequencing projects (3, 48).