TABLE 3.
Expression of malK-lacZ in BRE1219 with plasmids encoding MalT fragmentsa
| Plasmid | Size of MalT′ (aa) | β-Galactosidase activity (U mg−1) |
|---|---|---|
| Vector | 0.32 | |
| pACS20 | 325 | 0.31 |
| pACS21 | 250 | 0.32 |
| pACS22 | 200 | 0.25 |
| pACS23 | 150 | 0.23 |
| pACS24 | 100 | 0.27 |
The activity of the isogenic malk′ mutant strain BRE1162 is 4.88 U mg−1.