TABLE 3.
Expression of malK-lacZ in BRE1219 with plasmids encoding MalT fragmentsa
Plasmid | Size of MalT′ (aa) | β-Galactosidase activity (U mg−1) |
---|---|---|
Vector | 0.32 | |
pACS20 | 325 | 0.31 |
pACS21 | 250 | 0.32 |
pACS22 | 200 | 0.25 |
pACS23 | 150 | 0.23 |
pACS24 | 100 | 0.27 |
The activity of the isogenic malk′ mutant strain BRE1162 is 4.88 U mg−1.