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. Author manuscript; available in PMC: 2006 Sep 15.
Published in final edited form as: J Immunol. 2005 Sep 15;175(6):3715–3723. doi: 10.4049/jimmunol.175.6.3715

Figure 1.

Figure 1

Immunization with TRICOM-based vaccines in naive mice induced high avidity antigen-specific CTL. Panel A: C57BL/6 mice were vaccinated once with buffer (HBSS, inverted open triangles), rV-LacZ (open squares), rV-LacZ/B7-1 (closed circles) or rV-LacZ/TRICOM (closed triangles). After 30 days, splenocytes were harvested. β-gal-specific CD8+ T-cell precursor frequency and avidity were determined by intracellular IFN-β staining. Panel B: β-gal-specific CD8+ T-cell avidity as determined by cytolytic assay. Splenic T cells from rV-LacZ and rV-LacZ/TRICOM immunized mice were stimulated with irradiated B cells pulsed with 1μg/ml β-gal peptide for 5 days. CTL avidity was determined using lytic assay as described in Materials and Methods. rV-LacZ (open squares), rV-LacZ/TRICOM (closed triangles).