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. Author manuscript; available in PMC: 2006 Sep 15.
Published in final edited form as: J Immunol. 2005 Sep 15;175(6):3715–3723. doi: 10.4049/jimmunol.175.6.3715

Figure 4.

Figure 4

Effect of multiple booster vaccinations with rF-LacZ versus rF-LacZ/TRICOM on the expansion of antigen-specific memory T cells. Memory T cells were purified from rV-LacZ/TRICOM vaccinated Thy1.1 mice as described in Materials and Methods and in the legend to Fig. 2, and were adoptively transferred to C57BL/6 mice (Thy1.2). One week after adoptive transfer, mice were vaccinated three times with the indicated vaccines at 2-week intervals. Five days after second and third vaccinations, three mice from each group were sacrificed and splenocytes were prepared for monitoring expansion of CD8+/β-gal tetramer + T cells in the gated Thy1.1 T cell population.