TABLE 1.
Strain, plasmid, or primer | Description | Source |
---|---|---|
Strains | ||
H. pylori | ||
ATCC 43504 | Parent strain for all H. pylori strains | ATCCa |
ahpC::Kan type I | aphA3 insertion within ahpC | This study |
ahpC::Kan type II | aphA3 insertion within ahpC | This study |
napA::Cm | cat insertion within napA | This study |
ahpC::napA | ahpC napA double mutant | This study |
E. coli DH5α | Cloning strain | Lab stock |
Plasmids | ||
pBluescript KS+ | Cloning vector | Stratagene |
pKSΔS | pBluescript KS+ with destroyed SacI site | This study |
pAhpC | ahpC inserted into SmaI site of pBluescript KS+ | This study |
pAhpCKan | aphA3 inserted into AflII site of pAhpC | This study |
pNapA | napA cloned from ATCC 43504 into pBluescript KS+ | This study |
pNapAT1 | napA cloned from ahpC::Kan type I mutant | This study |
pNAΔS | napA cloned into pKSΔS | This study |
pNapA::Cm | cat inserted in SacI site of pNAΔS | This study |
Primers | ||
ahpCF | 5′ AGCCACGCCCAATAACGATG 3′ | IDTb |
ahpCR | 5′ TCGCCTTTTCTCCAACCTGCTG 3′ | IDT |
NapAF | 5′ AACCACTAAATTAAAGGGTAACGGC 3′ | IDT |
NapAR | 5′ CGCTGTAATCTCTCATGCTGGC 3′ | IDT |
NapART | 5′ ACACGATCGCATCCGCTTGC 3′ | IDT |
HP0630RT | 5′ AGAATGCCCGAACGCTTTGG 3′ | IDT |
ATCC, American Type Culture Collection.
IDT, Integrated DNA Technologies, Coralville, Coraville, Iowa.