TABLE 1.
Strains and plasmids
| Strain, plasmid, or primer | Description | Source |
|---|---|---|
| Strains | ||
| H. pylori | ||
| ATCC 43504 | Parent strain for all H. pylori strains | ATCCa |
| ahpC::Kan type I | aphA3 insertion within ahpC | This study |
| ahpC::Kan type II | aphA3 insertion within ahpC | This study |
| napA::Cm | cat insertion within napA | This study |
| ahpC::napA | ahpC napA double mutant | This study |
| E. coli DH5α | Cloning strain | Lab stock |
| Plasmids | ||
| pBluescript KS+ | Cloning vector | Stratagene |
| pKSΔS | pBluescript KS+ with destroyed SacI site | This study |
| pAhpC | ahpC inserted into SmaI site of pBluescript KS+ | This study |
| pAhpCKan | aphA3 inserted into AflII site of pAhpC | This study |
| pNapA | napA cloned from ATCC 43504 into pBluescript KS+ | This study |
| pNapAT1 | napA cloned from ahpC::Kan type I mutant | This study |
| pNAΔS | napA cloned into pKSΔS | This study |
| pNapA::Cm | cat inserted in SacI site of pNAΔS | This study |
| Primers | ||
| ahpCF | 5′ AGCCACGCCCAATAACGATG 3′ | IDTb |
| ahpCR | 5′ TCGCCTTTTCTCCAACCTGCTG 3′ | IDT |
| NapAF | 5′ AACCACTAAATTAAAGGGTAACGGC 3′ | IDT |
| NapAR | 5′ CGCTGTAATCTCTCATGCTGGC 3′ | IDT |
| NapART | 5′ ACACGATCGCATCCGCTTGC 3′ | IDT |
| HP0630RT | 5′ AGAATGCCCGAACGCTTTGG 3′ | IDT |
a
ATCC, American Type Culture Collection.
b
IDT, Integrated DNA Technologies, Coralville, Coraville, Iowa.