Figure. 1. Characterization of expression of selected ABC transporters in HEK293 transfectants.
Real time RT-PCR using SYBR green was performed on all of the cell lines utilized in this work. The mRNA expression values for MDR1 (ABCB1), MRP1 (ABCC1), MRP4 (ABCC4), MRP5 (ABCC5), and MRP8 (ABCC11) were determined for each cell line. Following normalization to GAPDH, the expression values for each transfectant was compared to the expression of each transporter within the parental HEK293 cells. The values represent the mean, and the error bars are standard deviation (n=4).