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. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: FEBS J. 2005 Sep;272(18):4725–4740. doi: 10.1111/j.1742-4658.2005.04888.x

Figure. 2. Sensitivity of control HEK293 and MRP4- and 5-expressing cells to thioguanine and NSC251820.

Figure. 2

Cytotoxicity assays were used to determine the sensitivity of control HEK293 (filled circles), MRP4-expressing HEK293/4.63 (open squares) and MRP5-expressing HEK293/5I (open triangles) to (A) thioguanine and predicted substrate of MRP4 based on data-mining NSC 251820 (C) as described previously [25]. The structure of NSC251820 is shown in (B). Cells (5.0 x 103 cells) were plated into 96 well plates, cultured overnight and then exposed to thioguanine for 72 h. Viable cells were determined by the Cell Counting Kit (CCK) technique as detailed in the Material and Methods section. The mean values from three independent experiments are shown with error bars as S.D.