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. 2002 Jun;184(12):3276–3286. doi: 10.1128/JB.184.12.3276-3286.2002

FIG. 3.

FIG. 3.

Metal selectivity of gene regulation in resuspension experiments. Cells were resuspended in minimal medium either containing no added Mn(II) or Fe(III) (open circles) or containing 5 μM Mn(II) (filled triangles), 10 μM Fe(III) (filled squares), or both (filled circles). Strains contained mrgA-cat-lacZ (A), katA-cat-lacZ (B), zosA-cat-lacZ (C), ahpC-cat-lacZ (D), hemA-cat-lac (E), perR-cat-lacZ (F), or fur-cat-lacZ (G). Samples were taken at the times indicated and assayed for β-Gal activity. The results shown are representative of at least three independent experiments; error bars represent the standard error of the mean of duplicate samples. Panel H is a Northern blot analysis of the fur transcript from cultures collected 3 h after resuspension in minimal medium containing the indicated metal ion supplementation.