TABLE I.
Influence of HuR levels on gene expression profiles of h VSMC upon PDGF stimulation for 8 h. This is a partial list of genes differentially expressed between Ctrl, siRNA- and HuR siRNA-transfected h VSMC populations, as evaluated by cDNA microarray analysis; the complete list of genes is available from the authors. The data were normalized using Z score transformation (see “Experimental Procedures”); the Z ratios indicate the difference in abundance between PDGF-treated cells and untreated cells; Z ratios ≥ 1.00 were considered as significantly elevated in the PDGF treatment groups. In parentheses, the number of genes is identified in each functional category.
| Genes and classification | Symbol | Z ratio |
GenBank™ accession no. | |
|---|---|---|---|---|
| Control siRNA | HuR siRNA | |||
| Proliferation (15) | ||||
| v-myb-like 2 | MYBL2 | 1.25 | 0.92 | NM_002466 |
| Cyclin-dependent kinase 2 | CDK2 | 1.00 | 0.77 | NM_001798 |
| v-raf viral oncogene homolog 1 | ARAF1 | 1.44 | 1.27 | NM_001654 |
| Replication factor C (activator 1) 5 | RFC 5 | 1.10 | 0.57 | NM_007370 |
| Metabolism (22) | ||||
| Arginase, type II | ARG2 | 1.27 | 0.54 | NM_001172 |
| Cationic amino acid transporter (y+) member 7 | SLC7A7 | 1.62 | 1.28 | NM_003982 |
| Spermidine/spermine acetyltransferase | SAT | 1.45 | 0.32 | NM_002970 |
| Oxysterol binding protein-like 2 | OSBPL2 | 1.05 | 0.48 | NM_144498 |
| Glutathione synthetase | GSS | 1.35 | 1.10 | NM_000178 |
| Procollagen C-endopeptidase enhancer | PCOLCE | 1.08 | 0.23 | NM_002593 |
| Diacylglycerol O-acyltransferase homolog 1 | DGAT 1 | 1.07 | 0.69 | NM_012079 |
| Ribophorin II | RPN2 | 1.26 | 1.00 | NM_002951 |
| Cytoskeleton and ECMa organization (18) | ||||
| Decorin | DCN | 1.03 | 0.17 | NM_001920 |
| Filamin A, α (actin binding protein 280) | FLNA | 1.41 | 1.05 | NM_001456 |
| Tara-like protein | HRIHFB2122 | 1.45 | 1.10 | NM_007032 |
| VAMP-associated protein B and C | VAPB | 1.34 | 0.88 | NM_004738 |
| Mitochondria (8) | ||||
| Translocase of inner mitochondrial membrane 17A | TIMM17A | 1.64 | 0.89 | NM_006335 |
| Solute carrier family 25, member 11 | SLC25A11 | 1.23 | 0.93 | NM_003562 |
| NADH dehydrogenase 1 β subcomplex | NDUFB8 | 1.91 | 1.69 | NM_005004 |
| Ribosome and translation (22) | ||||
| Ribosomal protein S6 | RPS6 | 1.43 | 0.63 | NM_001010 |
| Ribosomal protein L13 | RPL13 | 1.27 | 0.53 | NM_033251 |
| Eukaryotic translation initiation factor 4 γ, 1 | EIF4G1 | 1.02 | 0.71 | NM_004953 |
| Glutaminyl-tRNA synthetase | QARS | 1.20 | 0.68 | NM_005051 |
| Ribosomal protein S14 | RPS14 | 1.37 | 1.11 | NM_005617 |
| Eukaryotic translation initiation factor 4E-like 3 | EIF4EL3 | 1.46 | 1.27 | NM_004846 |
| Signal transduction (20) | ||||
| Calmodulin 2 (phosphorylase kinase, δ) | CALM2 | 1.04 | 0.58 | NM_001743 |
| Homer homolog 2 (Drosophila) | Homer-2 | 1.15 | 0.89 | NM_004839 |
| ADP-ribosylation factor 5 | ARF5 | 1.83 | 1.46 | NM_001662 |
| Guanine nucleotide binding protein β 2-like 1 | GNB2L1 | 1.21 | 1.00 | NM_006098 |
| Phospholipase C γ 2 | PLCG2 | 1.03 | 0.86 | NM_002661 |
| Transcription machinery and splicing (22) | ||||
| TAF9 TATA box binding protein-associated factor | TAF9 | 1.07 | 0.56 | NM_003187 |
| Splicing factor, arginine/serine-rich 7 | SFRS7 | 1.00 | 0.58 | NM_006276 |
| Zinc finger protein 144 (Mel-18) | ZNF144 | 1.30 | 0.99 | NM_007144 |
| Signal transducer and activator of transcription 3 | STAT3 | 1.02 | 0.80 | NM_139276 |
| Achaete-scute complex-like 1 (Drosophila) | ASCL 1 | 1.29 | 0.90 | NM_004316 |
| Miscellaneous (47) | ||||
| Nuclear RNA export factor 1 | NXF1 | 1.46 | 1.01 | NM_006362 |
| Small nuclear ribonucleoprotein polypeptide G | SNRPG | 1.14 | 0.87 | NM_003096 |
| Annexin A11 | ANXA 11 | 1.33 | 1.09 | NM_145869 |
| Proteasome subunit β type 4 | PSMB 4 | 1.32 | 0.77 | NM_002796 |
a
ECM, extracellular matrix.