TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild type | 10 |
| PAO4156 | gbuA9005 (=gbu-9005) | 16 |
| PAO4173 | gbuA9005 (=gbu-9005) gpu-9018 | N. Gotoh (Matsumoto collection) |
| PAO4508 | gbuR::Gm | This study |
| PAO4509 | PA1418::Gm | This study |
| PAO4511 | PA1419::Gm | This study |
| PAO4513 | PA1420::Gm | This study |
| PAO4518 | gbuA::Gm | This study |
| E. coli | ||
| DH5α | F− endA1 hsdR17(rk− mk+) supE44 thi-1 recA1 gyrA relA1 Δ(laclZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15] | Bethesda Research Laboratories |
| HB101 | supE44 hsdS20(rB−mB−) recA13 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-1 | 29 |
| S17-1 | pro thi hsdR Tpr Smr; chromosome::RP4-2 Rc::Mu-Km::Tn7 | 32 |
| XL1-Blue | endA1 gyrA96 thi-1 hsdR17 supE44 recA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tc)] | Stratagene |
| Plasmids | ||
| pEX18Ap | Apr, ColE1 replicon, oriT sacB | 13 |
| pME6013 | Tcr, pACYC177 and pVS1 replicons | 27 |
| pMMB67EH | Apr Cbr, RSF1010 derivative | 7 |
| pNIC6011 | Apr Cbr, pACYC177 and pVS1 replicons | 27 |
| pPS858 | Apr Gmr, ColE1 replicon carrying a Gmr cassette | 13 |
| pRK2013 | Kmr, ColE1 replicon, tra (RK2) | 5 |
| pUC118 | Apr, ColE1 replicon | 37 |
| pUC119 | Apr, ColE1 replicon | 37 |
| pYJ103 | Apr Cbr, pNIC6011 derivative carrying the 3.4-kb gbuRA region of the PAO1 chromosome | This study |