TABLE 2.
Inhibition of Salmonella OpdB
| Inhibitora | Inhibition constant
|
|
|---|---|---|
| Ki (μM) | kass (M−1 s−1) | |
| Reversible | ||
| para-Aminobenzamidine | 117.1 | |
| Benzamidine | 186.7 | |
| Leupeptin | 0.4 | |
| Antipain | 0.0081 | |
| Irreversible inhibitors | ||
| 3,4-Dichloroisocoumarin | 129.0 ± 12 | |
| 4-(2-Aminoethyl)benzenesulfonyl fluoride | 31.1 ± 8 | |
| Phenylmethane sulfonylfluoride | 0.2 ± 0.04 | |
a
No inhibition of activity of the wild-type enzyme was observed with iodoacetamide (1 mM), iodoacetate (1 mM), N-ethylmaleimide (1 mM), EDTA (1 mM), EGTA (1 mM), bestatin (10 μM), amastatin (10 μM), arphamenine A (10 μM), elastinal (10 μM), dynorphin A (10 μM), or dynorphin B (10 μM). The standard error of the Ki values was within 5% of the mean.