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. 2002 Jun;184(12):3203–3213. doi: 10.1128/JB.184.12.3203-3213.2002

TABLE 2.

Fold induction of promoter activity in indicated medium conditiona

Strain Medium condition Result for promoter fusion
mig-14::GFP
pmrC::GFP
pmrD::GFP
Mean peak fluorescence (aue) Fold induction Mean peak fluorescence (aue) Fold induction Mean peak fluorescence (aue) Fold induction
SL1344 (wild type)b 10 μM MgCl2 399 11.7 199 19.9 361 14.4
pH 5.8 70 2.1 34 3.4 35 1.4
PB 333 9.8 129 12.9 266 10.6
Protamine 218 6.4 107 10.7 214 8.6
PG-1 112 3.3 107 10.7 101 4.0
Triton X-100 57 1.7 13 1.3 45 1.8
SDS 56 1.6 39 3.9 53 2.1
phoP::Tn10c 10 μM MgCl2 6 1 1 1 4 1
pH 5.8 6 1 5 5 3 0.75
PB 26 4.3 1 1 9 2.25
Protamine 5 0.8 1 1 5 1.25
PG-1 4 0.7 1 1 4 1
Triton X-100 6 1.0 1 1 4 1
SDS 6 1.0 1 1 5 1.25
pmrA::Kand 10 μM MgCl2 798 20.5 1 1 509 11.6
pH 5.8 67 1.7 1 1 42 1
PB 805 20.6 1 1 212 4.8
Protamine 286 7.3 1 1 222 5.0
PG-1 104 2.7 1 1 158 3.6
Triton X-100 56 1.4 1 1 58 1.3
SDS 54 1.4 1 1 74 1.7
a

Mean peak fluorescence values are for the populations grown under the condition indicated. Fold inductions were calculated by dividing the mean peak fluorescence values for growth under the condition indicated by the mean peak fluorescence values for growth in 10 mM MgCl2 at pH 7.4 (values given in footnotes b, c, and d).

b

Graphic representations of these results are shown in Fig. 1. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 34; for pmrC::GFP, 10; and for pmrD::GFP, 25.

c

Graphic representations of these results are shown in Fig. 2. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 6; for pmrC::GFP, 1; and for pmrD::GFP, 4.

d

Graphic representations of these results are shown in Fig. 3. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 39; for pmrC::GFP, 1; and for pmrD::GFP, 44.

e

au, arbitrary units.