TABLE 2.
Fold induction of promoter activity in indicated medium conditiona
Strain | Medium condition | Result for promoter fusion
|
|||||
---|---|---|---|---|---|---|---|
mig-14::GFP
|
pmrC::GFP
|
pmrD::GFP
|
|||||
Mean peak fluorescence (aue) | Fold induction | Mean peak fluorescence (aue) | Fold induction | Mean peak fluorescence (aue) | Fold induction | ||
SL1344 (wild type)b | 10 μM MgCl2 | 399 | 11.7 | 199 | 19.9 | 361 | 14.4 |
pH 5.8 | 70 | 2.1 | 34 | 3.4 | 35 | 1.4 | |
PB | 333 | 9.8 | 129 | 12.9 | 266 | 10.6 | |
Protamine | 218 | 6.4 | 107 | 10.7 | 214 | 8.6 | |
PG-1 | 112 | 3.3 | 107 | 10.7 | 101 | 4.0 | |
Triton X-100 | 57 | 1.7 | 13 | 1.3 | 45 | 1.8 | |
SDS | 56 | 1.6 | 39 | 3.9 | 53 | 2.1 | |
phoP::Tn10c | 10 μM MgCl2 | 6 | 1 | 1 | 1 | 4 | 1 |
pH 5.8 | 6 | 1 | 5 | 5 | 3 | 0.75 | |
PB | 26 | 4.3 | 1 | 1 | 9 | 2.25 | |
Protamine | 5 | 0.8 | 1 | 1 | 5 | 1.25 | |
PG-1 | 4 | 0.7 | 1 | 1 | 4 | 1 | |
Triton X-100 | 6 | 1.0 | 1 | 1 | 4 | 1 | |
SDS | 6 | 1.0 | 1 | 1 | 5 | 1.25 | |
pmrA::Kand | 10 μM MgCl2 | 798 | 20.5 | 1 | 1 | 509 | 11.6 |
pH 5.8 | 67 | 1.7 | 1 | 1 | 42 | 1 | |
PB | 805 | 20.6 | 1 | 1 | 212 | 4.8 | |
Protamine | 286 | 7.3 | 1 | 1 | 222 | 5.0 | |
PG-1 | 104 | 2.7 | 1 | 1 | 158 | 3.6 | |
Triton X-100 | 56 | 1.4 | 1 | 1 | 58 | 1.3 | |
SDS | 54 | 1.4 | 1 | 1 | 74 | 1.7 |
Mean peak fluorescence values are for the populations grown under the condition indicated. Fold inductions were calculated by dividing the mean peak fluorescence values for growth under the condition indicated by the mean peak fluorescence values for growth in 10 mM MgCl2 at pH 7.4 (values given in footnotes b, c, and d).
Graphic representations of these results are shown in Fig. 1. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 34; for pmrC::GFP, 10; and for pmrD::GFP, 25.
Graphic representations of these results are shown in Fig. 2. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 6; for pmrC::GFP, 1; and for pmrD::GFP, 4.
Graphic representations of these results are shown in Fig. 3. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 39; for pmrC::GFP, 1; and for pmrD::GFP, 44.
au, arbitrary units.