Fig. 2.

RT-PCR and Northern blot analyses of total A. gambiae RNA. A-F): RT-PCR products were amplified from female and male adult A. gambiae total RNA using the following combinations of primers: A) dsx1f and dsx1r; B) dsx2f and dsx1r; C) dsx1f and dsx2r; D) dsx3f and dsx3r; E) dsxef and dsxer; F) S7for and S7rev (which amplify the housekeeping ribosomal S7 gene as a control). For primer locations see Fig. 1. G) Northern blot analysis was performed on total RNA (10μg) extracted from male and female A. gambiae adults. Lane 1: female adult; lane 2: male adult. Upper panel: hybridization performed with Probe 5’; lower panel: standardization with probe S7. The molecular size is shown in kb.