Table 1.

Splice acceptor sequences of Agdsx introns. The intron/exon boundaries at the 3′ of intron 2, 3, 4 and 5 were amplified from genomic A. gambiae DNA, cloned and sequenced to analyze the 3′ splice acceptor sites. The number of pyrimidines in the 12 bp preceding the 3′ acceptor site (NYAG) is indicated. The consensus number of pyrimidines (8.69) is derived from the analysis of 215 A. gambiae splice acceptor sites. Intron 4 represents the intron preceding the female-specific exon 5 and its polypyrimidine stretch does not deviate significantly from the consensus, suggesting it may not be a weak acceptor site.

	Intron	Acceptor Sequences	Number of Pyr
Agdsx	2	TTGCTCTCCTTT TCAG	11
	3	TTCCGCCCCGTT TCAG	10
	4	TTTATGTTTAAC ACAG	8
	5	TGTAACCCCCAA AAAG	7
Consensus			8.69 ± 2.07