TABLE 2.

Effect of tldD and/or tldE deletion on the transformation efficiency of a plasmid expressing the CcdA41 and CcdB proteins^a

Strain	Transformation efficiency
	pULB2208		pULB2212		pULB2250 (− IPTG)	pULB2705 (− IPTG)
	− IPTG	+ IPTG	− IPTG	+ IPTG
SG22622	<0.001	1.1	<0.001	<0.001	<0.001	0.9
SG22622 ΔtldD	1	1.1	<0.001	<0.001	<0.001	0.9
SG22622 ΔtldE	0.9	0.9	<0.001	<0.001	<0.001	1.2
SG22622 ΔtldD ΔtldE	1.4	1.3	<0.001	<0.001	<0.001	1.7
SG22622 gyrA462	1.1	1.0	0.8	0.9	0.8	0.8

^aIsogenic strains were transformed with equal amounts of plasmid DNA expressing CcdA41 and CcdB (pULB2208), CcdB alone (pULB2212 and pULB2250), or CcdA and CcdB (pULB2705). Transformation efficiency was calculated by dividing the number of transformants obtained with the plasmid bearing the ccd genes by the number of transformants obtained with the pKK223-3 control vector. Similar results were obtained at least three times.