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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Circ Res. 2004 Apr 22;94(10):1310–1317. doi: 10.1161/01.RES.0000129180.13992.43

Figure 1.

Figure 1

Prolonged HCMV infection induced endothelial apoptosis. A, HCMV infection resulted in substantial cytopathicity in endothelial cells. HAECs were infected with VHL/E strain of HCMV for 9 days and changes in morphology and viability were observed under light microscope. B, Detection of endothelial apoptosis in HCMV-infected HAECs. VHL/E-infected HAECs (day 9 pi) were stained with anti-IE (HCMV immediate early antigen) antibody to indicate infected cells (red), TUNEL (green) for apoptosis, and DAPI (blue) for nuclei. Merged image shows colocalization. C, Time-dependent apoptosis of HCMV-infected HAECs. HAECs were infected with VHL/E for indicated time periods; cells were stained with anti-IE antibody (red), TUNEL (green), and DAPI (blue). Merged images show colocalization. D, Time-dependent death curves of VHL/E-infected HAECs. TUNEL-positive cells and total cells were counted in 5 fields per coverslip. Results were expressed as the average percentage of TUNEL-positive cells over total cells.