TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or construction | Source or reference |
|---|---|---|
| Strains | ||
| MM2089 | SL1344 wild type | 24 |
| MM2211 | UK1 oxyR::Tn10 (JA4023) | J. Fostera |
| MM2212 | SF1 iroA::MudJ zbf-5126::Tn10 fur-9 (JA3199) | J. Foster |
| MM2625 | 14028 Δfur41::kan (JS219) | This study |
| MM2626 | 14028 ΔmntR51::cam (JS220) | This study |
| MM2525 | SL1344 oxyR::Tn10; P22 from MM2211 into SL1344 | This study |
| MM2526 | SL1344 zbf-5126::Tn10 fur-9; P22 from MM2212 into SL1344 | This study |
| MM2635 | SL1344 Δfur41::kan; P22 from MM2625 into SL1344 | This study |
| MM2634 | SL1344 ΔmntR51::cam; P22 from MM2626 into SL1344 | This study |
| MM2656 | SL1344 Δfur41::kan ΔmntR51::cam; P22 from MM2625 into MM2634 | This study |
| MM2507 | MM2089/pMLZ104 | This study |
| MM2529 | MM2525/pMLZ104 | This study |
| MM2530 | MM2526/pMLZ104 | This study |
| MM2646 | MM2635/pMLZ104 | This study |
| MM2645 | MM2634/pMLZ104 | This study |
| MM2654 | MM2656/pMLZ104 | This study |
| MM2616 | MM2089/pDGK261 | This study |
| MM2617 | MM2089/pDGK262 | This study |
| MM2618 | MM2089/pDGK263 | This study |
| MM2762 | MM2646/pDGK262 | This study |
| MM2763 | MM2646/pDGK263 | This study |
| MM2759 | MM2645/pDGK262 | This study |
| MM2760 | MM2645/pDGK263 | This study |
| Plasmids | ||
| pMLZ104 | pFZY1 mini-F′ plasmid derivative, with an mntH::lacZYA fusion controlled by wild-type mntH promoter on an EcoRI-HindIII insert | 24 |
| pDGK227 | pBluescript II SK(+) containing EcoRI-HindIII mntH promoter insert from pMLZ104 | 24 |
| pDGK246 | Intermediate in construction of pDGK247 (see Materials and Methods) | This study |
| pDGK247 | pBluescript II SK (+) containing EcoRI-HindIII mntH promoter insert containing SphI, SalI, and BamHI sites between putative cis-acting elements in mntH promoter | This study |
| pDGK251 | pDGK247 with P22 Pant sequence and XbaI site in place of PmntH OxyR-binding motif | This study |
| pDGK252 | pDGK247 with P22 Pant sequence and SacI site in place of PmntH Fur-binding motif | This study |
| pDGK253 | pDGK247 with P22 Pant sequence and KpnI site in place of PmntH MntR-binding motif | This study |
| pDGK261 | pMLZ104 with P22 Pant sequence and XbaI site in place of PmntH OxyR-binding motif | This study |
| pDGK262 | pMLZ104 with P22 Pant sequence and SacI site in place of PmntH Fur-binding motif | This study |
| pDGK263 | pMLZ104 with P22 Pant sequence and KpnI site in place of PmntH MntR-binding motif | This study |
a
Department of Microbiology, University of South Alabama, Mobile.