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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2005 Aug 4;280(43):36221–36227. doi: 10.1074/jbc.M506727200

Figure 2. Western Blot Analysis of Dmt1 Protein Expression in the Duodenum and Jejunum of Iron-Deficient Rats.

Figure 2

Intestinal mucosa was harvested from groups of rats and brush-border membrane vesicle proteins were purified by the well-established Mg++ precipitation method. SDS/PAGE followed by immunoblotting was subsequently performed with a 1:4000 dilution of the Dmt1 antiserum. As shown, significant induction of DMT1 was seen in 6-week and 12-week-old iron-deficient rats, and a modest induction was also detected in the jejunum of a group of 12-week-old iron-deficient rats.