TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or Reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD ΔlacU169 rpsL thi-1 | 8 |
| MV198 | MC4100 λMV195 lysogen, [agn(8785-9525)′-lacZ] | 15 |
| MV201 | MV198 oxyR:: Ω(Spec) | 15 |
| MV202 | MV198 dam-13::Tn9 | 15 |
| MV203 | MV198 pTP166 | 15 |
| MV253 | MC4100 pTP166 | This study |
| MV289 | MC4100 [agn(8785-9525)(AATC-III)′-lacZ]; λMV284 (λRS45-pMV124 recombinant) lysogen | This study |
| MV301 | MC4100 λMV139 lysogen, [agn(9212-9525)′-lacZ] | This study |
| MV310 | MC4100 [agn(8785-9525)(AATC-II)′-lacZ]; λMV294 (λRS45-pMV130 recombinant) lysogen | This study |
| MV336 | MC4100 [agn(8785-9525)(GATG-I)′-lacZ]; λMV330 (λRS45-pMV137 recombinant) lysogen | This study |
| MV348 | MC4100 λMV139 lysogen [agn(9296-9525)′-lacZ] | This study |
| MV353 | MC4100 [agn(8785-9525)(GATG-I; AATC-II, AATC-III)′-lacZ]; λMV142 (λRS45-pMV142 recombinant) lysogen | This study |
| MV365 | MC4100 λMV141 lysogen, [agn(9248-9525)′-lacZ] | This study |
| MV383 | MV203 katF13::Tn10 | This study |
| MV415 | RZ201 λMV198 lysogen (F− Δ(lac-proAB)x111ara thi rpsL CSH26 recA Strr) | 21, this study |
| MV416 | RZ6520 λMV198 lysogen (F− Δ(lac-proAB)x111ara thi rpsL CSH26 recA StrrrpoD800 zgh-3075:Tn10) | 21, this study |
| MV425 | MV415 pTP166 | |
| MV426 | MV416 pTP166 | This study |
| MV441 | MC4100 λMV145 lysogen (agnK1′-lacZ); contains E. coli K1 agn43 DNA homologous to nt 8785-9525 from E. coli K-12 | This study |
| MV442 | MV441 dam-13::Tn9 | This study |
| MV444 | MV441 oxyR:: Ω(Spec) | This study |
| MV496 | pMV151 in XL-1 Blue | This study |
| MV497 | pMV151 in GM2929 (dam-13::Tn9 dcm-6 hsdR2 recF143 McrA− McrB−) | This study; Marinus (unpublished) |
| MV500 | MC4100 λMV149 lysogen, [agn(8785-9312)′-lacZ] | This study |
| MV556 | MC4100 λMV167 lysogen, [agn(9212-9312)′-lacZ] | This study |
| UM122 | HfrH thi katF13::Tn10 | 30 |
| Plasmids | ||
| pTP166 | pBR322 with dam under Tac promoter control | 28 |
| pRLG593 | pKM2 flanked by terminator sites (pRLG770) containing an EcoRI-HindIII fragment with the lacUV5 promoter region | 35 |
| pMV124 | pRS550 with agn(8785-9525) insert containing a point mutation at nt 9283 resulting in agn(AATC-III) | 39, this study |
| pMV130 | pRS550 with agn(8785-9525) insert containing a point mutation at nt 9270 resulting in agn(AATC-II) | 39, this study |
| pMV137 | pRS550 with agn(8785-9525) insert containing a point mutation at nt 9254 resulting in agn(GATG-I) | 39, this study |
| pMV142 | pRS550 with agn(8785-9525) insert containing point mutations at nt 9254, 9270, and 9283 resulting in agn(GATG-I; AATC-II, AATC-III) | 39, this study |
| pMV151 | pRLG770 with an EcoRI-HindIII fragment containing agn43(8785-9389). | 35, this study |