Figure 1.

Epithelial migration and outgrowth of corneo-limbo-conjunctival explant cultures. A: Representative explants on day 5 exhibited that the pigment line at the limbal epithelium remained stationary when cultured in submerged modes. In contrast, the limbal pigment line migrated toward the sclera (white portion) when cultured in the AL mode (arrow). B: Continuous migration of the limbal pigment line of an AL explant toward the sclera (black arrow) from day 3 to day 7. C and D: The surface area of epithelial outgrowth on the membrane in AL explants was significantly more than that of submerged (P < 0.001). Explant location was marked by an asterisk in C.