FIG. 3.

Western blot analysis of intra- and extracellular proteins. Shown is immunodetection (anti-YscJ, -YopN, and -YopE polyclonal antibodies or anti-YopB monoclonal antibody) of proteins from whole-cell extracts (C) (lanes 1, 3, 5, and 7) and culture supernatants (SN) (lanes 2, 4, 6, and 8) of Y. enterocolitica MRS40(pYV40) (wild type [WT]) (lanes 1 to 4) or SBY40(pYV40) (flhDC) (lanes 5 to 8) grown for 6 h at RT (lanes 1, 2, 5, and 6) or 2 h at RT and 4 h at 37°C (lanes 3, 4, 7, and 8) in BHI-Ox. The number of bacteria loaded or the number of bacteria corresponding to the volume of supernatant loaded is given below the panels.