TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant featuresa | Reference or source |
|---|---|---|
| Y. enterocolitica strains and pYV plasmids | ||
| MRS40(pYV40) | Wild type | 33 |
| MRS40(pAB4052) | yopE52 | 29 |
| SBY40(pYV40) | flhDΔ44-119 flhCΔ1-124 | This work |
| SBY40(pAB4052) | yopE52 flhDΔ44-119 flhCΔ1-124 | This work |
| KNG22703(pSW2276) | yscNΔ169-177 | 40 |
| SBY22703(pSW2276) | yscNΔ169-177 flhDΔ44-119 flhCΔ1-124 | This work |
| W22703(pGC1153) | virF::pGCS904 | 21 |
| MI1024(pYV1024) | fliA::aphA3 | 15 |
| Other plasmids | ||
| pBSSK(−) | Cloning vector | Stratagene |
| pGCS757 | virF-cat transcriptional fusion in pTZ19R plus oriTRK2 | 5 |
| pGEX-KG | Cloning vector generating translational fusions with GST | Pharmacia Biotech |
| pGY10 | 4.3-kb EcoRI fragment containing flhDC genes of Y. enterocolitica 8081v cloned in pTM100 | 44 |
| pKNG101 | Suicide vector; oriR6Kpir oriTRK2 | 16 |
| pPW107 | pBC19R plus kanamycin resistance cassette | P. Wattiau and G. R. Cornelis, unpublished data |
| pSBY10 | flhDC mutator obtained by cloning the mutated flhD′C′ allele from pSBY9 digested with SalI and XbaI in the same sites of pKNG101 | This work |
| pSBY5 | pGEX-KG producing a hybrid GST-YscJ′ protein (191-bp fragment of yscJ [nucleotides 23-214]) | This work |
| pSBY6 | PCR-amplified flhD and flhC cloned opposite plac in pBluescript SK(−) | This work |
| pSBY7 | StyI deletion in pSBY6 | This work |
| pSBY9 | Cloned kanamycin resistance cassette in the StyI site of pSBY7 blunted with the Klenow fragment of polymerase | This work |
| pSI55 | plac, sycE (bp −25 to stop), and yopE (bp −21 to stop) cloned in the same orientation in pTM100 | I. Stainier and G. R. Cornelis, unpublished data |
| pTM100 | pACYC184 plus oriTRK2 (medium-copy-number, mobilizable vector) | 26 |
a
Deletions indicate the base pairs deleted from the gene; e.g., in flhDΔ44-119, bp 44 to 119 are deleted from flhD. oriTRK2, origin of transfer of plasmid RK2; oriR6K, origin of replication of plasmid R6K.