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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Exp Cell Res. 2005 Oct 11;311(2):251–264. doi: 10.1016/j.yexcr.2005.09.005

Figure 1.

Figure 1

Expression of steroid/nuclear receptors in mES cells. RNA was prepared from cells cultured as described in Materials and Methods and RT-PCR performed using primers and conditions listed in Table I. Samples from mES cells maintained in the undifferentiated state by addition of LIF to the culture media are labeled Undif (+LIF). Differentiation was induced by LIF withdrawal for the time indicated in either plated cells or EBs. A. Detection of mRNA for a variety of steroid/nuclear receptors in CCE mES cells. B. Real time quantitative PCR analysis for PR expression during LIF withdrawal in CCE mES cells. Fold increases are 1.6 +/− 0.4 at 4h, 3.0 +/− 0.4 at 48h, and 16.3 +/− 1.4 at 96h. Fold increases are 1.6 +/− 0.4 at 4h, 3.0 +/− 0.4 at 48h, and 16.3 +/− 1.4 at 96h. C. Detection of mRNA for a series of mES cell differentiation markers in CCE cells. D. PR and cell differentiation marker expression patterns during differentiation of D3 mES cells. GAPDH is used as a normalization control.