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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Exp Cell Res. 2005 Oct 11;311(2):251–264. doi: 10.1016/j.yexcr.2005.09.005

Figure 3.

Figure 3

Short timecourse of PR expression in mES cells upon differentiation induction. RNA was prepared from cells cultured as described in Materials and Methods and RT-PCR performed using primers and conditions listed in Table I. Samples from mES cells maintained in the undifferentiated state by addition of LIF to the culture media are labeled Undif (+LIF). Differentiation was induced by either LIF withdrawal, addition of RA, or both for the times indicated in plated cells. The expression of a series of mES cell differentiation markers is also shown. GAPDH is used as a normalization control.