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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Exp Cell Res. 2005 Oct 11;311(2):251–264. doi: 10.1016/j.yexcr.2005.09.005

Figure 6.

Figure 6

In mES cells, differentiation, not PR ligand, regulates the expression of three genes that are progesterone-responsive in other systems. RNA was prepared from cells cultured as described in Materials and Methods and RT-PCR performed using primers and conditions listed in Table I. Samples from mES cells maintained in the undifferentiated state by addition of LIF to the culture media are labeled Undif (+LIF). Differentiation was induced by LIF withdrawal for 4 days in plated cells (−LIF) or 8 days in EBs. Cells were treated with vehicle control (V), 200 nM progesterone (P), or 1 μM RU486 (R).