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Calculated and experimental charge states of 11 peptides formed by electrospray ionization from methanol-water-acid solutionsa
| Measured charge state
|
Calculated maximum charge state
|
||||
|---|---|---|---|---|---|
| Peptidea | Number of basic sites | Average | Maximum | Original model | Flexible terminus modification |
| G6 | 1 | 1.0 | 1 | 1 | 1 |
| R3 | 4 | — | 3 | 2 | 3 |
| (RG)3 | 4 | 2.8 | 3 | 3 | 3 |
| (RG)6 | 7 | 4.6 | 5 | 4 | 4 |
| (RRGG)3 | 7 | 4.0 | 5 | 4 | 5 |
| R6G6 | 7 | 4.0 | 4 | 4 | 4 |
| Re | 7 | 3.9 | 4 | 3 | 4 |
| (RG)10 | 11 | 4.0 | 5 | 6 | 6 |
| (RĀ)9R | 11 | 4.7 | 6 | 7 | 7 |
| (RG)15 | 16 | 7.9 | 9 | 9 | 9 |
| (RĀ)14R | 16 | 8.3 | 10 | 10 | 10 |
