TABLE 1.
E. coli K-12 strains, plasmids, and bacteriophages
| Strain, plasmid, or phage | Origin | Relevant genotype or phenotype | Source or reference |
|---|---|---|---|
| Strains | |||
| MC4100 | F Δ(argF-lac)U169 | Laboratory stock | |
| PM6 | MC4100 | modC modE::Kanr | 20 |
| PM8 | MC4100 | modE::kanr | 20 |
| Plasmids | |||
| pR415 | pBR322 | lacZ+lacY+lacA+ Ampr | 30 |
| pPM6 | pACYC184 | modE+ Cmr | 20 |
| pPM9 | pACYC184 | modE∗; encodes a molybdate independent modE allele, Cmr | 20 |
| pHW2 | pRS415 | napF-lacZ operon fusion, Ampr | Laboratory stock |
| pPM58 and -59 | pRS415 | napF-lacZ; operon fusions with upstream deletions in napF promoter region, Ampr | This study |
| pPM69 | pPM58 | napF-lacZ; pPM58 with upstream region cloned back in correct orientation, Ampr | This study |
| pPM70 | pPM58 | napF-lacZ; pPM58 with upstream region cloned back in reverse orientation, Ampr | This study |
| pPM71 | pPM69 | napF-lacZ; pPM69 105-bp insert from the cat gene cloned in the EcoRI site, Ampr | This study |
| pPM54 and -55 | pRS415 | napF-lacZ; operon fusions with mutations in the ModE binding motif, Ampr | This study |
| Phages | |||
| λRS45 | lacZ′ lacY+lacA+ | 30 | |
| λHW2 | λRS45 | Φ(napF-lacZ) (operon fusion) | Laboratory stock |
| λPM58 and -59 | λRS45 | Φ (napF-lacZ) (operon fusions with upstream deletions in napF promoter region) | This study |
| λPM69 | λPM58 | Φ(napF-lacZ) (λPM58 with upstream region cloned back in correct orientation) | This study |
| λPM70 | λPM58 | Φ(napF-lacZ) (λPM58 with upstream region cloned back in reverse orientation) | This study |
| λPM71 | λPM69 | Φ(napF-lacZ) (λPM69 with 105-bp insert from the cat gene cloned in the EcoRI site) | This study |
| λPM54 and 55 | λRS45 | Φ(napF-lacZ) (operon fusions with mutations in the ModE binding site) | This study |