TABLE 2.
Effect of rpoS-rho interaction on proU P1-lac expressiona
| Strain | Genotype | β-Galactosidase sp act with the following plasmid and conditions: | |||||
|---|---|---|---|---|---|---|---|
| pHYD275 (E. coli P1-lac) | pHYD373 (S. enterica P1-lac) | ||||||
| No NaCl, 30°C | NaCl, 30°C | No NaCl, 10°C | No NaCl, 30°C | NaCl, 30°C | No NaCl, 10°C | ||
| GJ862 | rpoS+rho+ | 63 | 275 | 962 | 4 | 4 | 54 | 
| GJ863 | rpoS+rho | 126 | 322 | 885 | 51 | 69 | 463 | 
| GJ829 | rpoS::IS10 rho+ | 7 | 14 | 56 | 1 | 2 | 2 | 
| GJ830 | rpoS::IS10 rho | 157 | 467 | 1,360 | 26 | 104 | 849 | 
Cultures were grown to mid-exponential phase in trimethoprim-supplemented K-tryptone medium without or with 0.3 M NaCl at 30 or 10°C for β-galactosidase assays. Enzyme specific activities are reported in Miller units (22).