TABLE 3.
Ara-induced expression of proU P1 from pHYD275 in pHYD408 derivatives of rpoS rho+ strainsa
| Strain | Chromosomal rpoS genotype | β-Galactosidase sp act with:
|
|
|---|---|---|---|
| Glu | Ara | ||
| GJ2758 | rpoS::IS10 | 17 | 783 |
| GJ2756 | rpoS::Tn10 | 11 | 447 |
| GJ2778 | ΔrpoS | 10 | 263 |
Cultures were incubated in LB medium supplemented with trimethoprim, ampicillin, and either Glu or Ara at 30°C until 1 h after entry into stationary phase for β-galactosidase assays. Enzyme specific activities are reported in Miller units (22).