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. 2006 Jan 26;116(2):436–446. doi: 10.1172/JCI24684

Figure 8.

Figure 8

Effect of NO2– on function of cultured human airway epithelia. (A) Culture preparations of human CF airway epithelium (duplicate preparations, n = 3) were exposed apically to 2 μl of varying concentrations of NO2–. After 24 hours, lactate dehydrogenase activity in the basolateral media was measured to monitor cytotoxicity. (B) CF airway epithelial cultures were mounted in Ussing chambers and treated with 2 μl of liquid containing NO2– at varying concentrations. Transepithelial short circuit current (Isc, in μA/cm2) was measured to monitor change in bioelectric properties. (C) CF airway epithelial cultures (triplicate preparations, n = 4), were treated luminally with 100 μl Krebs bicarbonate Ringer buffer containing 2% blue dextran and supplemented with either 15 mM NaCl (control) or NO2– for 24 hours. Transepithelial water flux (Jv) was calculated by measuring blue dextran concentration optically after 24 hours in microaliquots of sampled luminal liquid. (D) IL-8 release assay using primary cultures of CF airway epithelia (n = 4) exposed to 15 mM NO2– compared with control cultures. (E) Determination of NO2– half life on the surface of airway epithelia. The 3 different concentrations of NO2– were applied to airway epithelial cell monolayers, and NO2– levels were assayed at the indicated intervals (n = 3 for each measurement). P = NS for all comparisons (ANOVA).