Table 3.
Infection of DC (immature and matured with CD40L) with rF-TRICOM enhances avidity of CTL
Treatment of DC | Avidity (M) of CTL | Avidity vs DC (fold)* | Avidity vs CD40L-matured DC (fold)** |
---|---|---|---|
DC | 2.29 x 10−8 | 1.0 | −2.3 |
DC infected with FP-WT | 1.31 x 10−8 | 1.7 | −1.3 |
DC infected with rF-TRICOM | 3.5 x 10−10 | 65.4 | 28.0 |
DC matured with CD40L | 9.81 x 10−9 | 2.3 | 1.0 |
DC matured with CD40L and infected with FP-WT | 8.12 x 10−9 | 2.8 | 1.2 |
DC matured with CD40L and infected with rF-TRICOM | 7.0 x 10−11 | 327.0 | 140.0 |
DC were generated with GM-CSF and IL-4 with or without maturation with CD40L and/or infected with rF-TRICOM or wild-type vector FP-WT, as described in Materials and Methods.
Numbers are the fold increase in avidity of activated T cells, as compared with those activated with DC generated with GM-CSF and IL-4.
Numbers are the fold increase or decrease in avidity of activated T cells, as compared with those activated with DC generated with GM-CSF and IL-4 and matured with CD40L.