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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2004 Apr 2;279(25):26735–26747. doi: 10.1074/jbc.M401766200

Fig. 11. The autocrine/paracrine actions of IL-6 are necessary for trans-10, cis-12 CLA-mediated induction of MEK/ERK signaling.

Fig. 11

Cultures of SV cells containing newly differentiated human adipocytes were serum starved for 24 h and A, treated with human recombinant IL-6, IL-8, or TNF-α (all 100 ng/ml) for 0–30 min; or B, pretreated with or without neutralizing antibodies (Ab) for IL-6 (50 μg/ml), IL-8 (50 μg/ml), or TNF-γ (20 μg/ml) and subsequently treated with either a BSA vehicle (B) or 30 μm trans-10, cis-12 CLA for an additional 24 h or recombinant IL-6, IL-8, or TNF-α for an additional 30 min. Total cell extracts were immunoblotted for P-ERK1/2. Data shown are representative of two to three independent experiments for each panel.

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